|
KMID : 0366119750030020063
|
|
Korean Journal of Applied Microbiology & Bioengineering
1975 Volume.3 No. 2 p.63 ~ p.68
|
|
|
Studies on the Biological Active Substance Produced by a Strain of Streptomyces sp.
|
|
|
|
|
Abstract
|
|
|
A biological active substance was isolated from the cultured medium of Streptomyces sp. and its biochemical characteristics were investigated. Isolation process of the substance was as follows; the?pH of filterate of the cultured medium was adjusted to 3.0 with N-hydrochloric acid and saturated with sodium chloride, then chloroform was added to this filterate in one fifth portions and stirred vigorously. After extracting the active substance with chloroform in 3 stages, the chloroform layer combined and evaporated after dehydrating with sodium sulfate.
The substance was found to be toxic to various fresh water fishes; the lethal dose for an average size Pseudorasbora parva T. et. S. was 50μg per ml. In the acidic condition, the toxicity of the substance remained fora long time, while in the alkaline state, the toxicity was decreased very.fast. This substance was found to be stable to organic solvents, but labile to heat treatment. The maximal revival time of Pseudorasbora parva T. et S. was about 20 minutes in 25 μg/ml of the substance solution.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|